Massively parallel pyrosequencing in HIV research.
نویسندگان
چکیده
Several methods for massively parallel pyrosequencing have recently been commercialized. As an example, consider the use of the 454 Life Sciences pyrosequencing method for metagenomic analysis of woolly mammoth DNA [1,2]. DNA from a mammoth carcass was purified and fragmented, and DNA linkers were ligated to the free ends. DNAs were then denatured and strands annealed to beads conjugated with oligonucleotides complementary to the linker sequences. This step is carried out with very low DNA concentrations so that on average only one strand binds to each bead. Bead-bound DNA is then PCR amplified in an oil–water emulsion, where each water droplet in the emulsion contains on average a single bead. The amplified DNA strands anneal to the beads,
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عنوان ژورنال:
- AIDS
دوره 22 12 شماره
صفحات -
تاریخ انتشار 2008